Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 7 - Jul 12
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse TMX2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Thioredoxin Related Transmembrane Protein 2 (TMX2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Thioredoxin Related Transmembrane Protein 2 (TMX2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Thioredoxin Related Transmembrane Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Thioredoxin-related transmembrane protein 2 (TMX2) is encoded by the TMX2 gene, a member of the endoplasmic reticulum (ER) protein disulfide isomerase (PDI) family. This protein catalyzes protein folding and thiol-disulfide exchange reactions. The encoded protein has an N-terminal ER signal sequence, a catalytically active thioredoxin domain, a transmembrane domain, and a C-terminal ER retention sequence. This protein is enriched in mitochondrial-associated membranes of the ER through palmitoylation of two membrane-exposed cysteines. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.2 ★★★★★
Based on 1996 reviews
Sort
Product Reviews
★★★★★ 5
Comfortable and Great Value Office Chair
This office chair exceeded my expectations for the price. The assembly was quick and easy, and the chair feels sturdy once put together. The seat cushion is comfortable for extended use, while the mesh back helps keep airflow moving throughout the day.
I also like the adjustable lumbar support and flip-up armrests, which make it easy to customize and save space when not in use. It rolls smoothly, looks professional, and provides great comfort for working from home. Overall, an excellent value and a solid choice for a home office.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 12, 2026
★★★★★ 5
Very comfortable
Great chair, simple assembly that took ~20 minutes. Very smooth height adjustments, even has a rocking function. Very sturdy and the build quality seems good. Breathable fabric for the back and good quality leather for the seats.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Great value high quality
The chair is very comfortable, even during long hours of work. The cushioning provides great support, and the ergonomic design helps maintain a good posture throughout the day. I also appreciate the adjustable features (height, armrests, and tilt), which make it easy to customize to my preference.
Overall, this is a great value for the price. If you’re looking for a reliable, comfortable office chair for daily use, I would definitely recommend this one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 20, 2026
★★★★★ 5
Great chair with one caveat!!
I ordered this chair to replace my previous computer chair that lacked back support and was generally uncomfortable, and I am very glad I did!!
I was looking for this style of chair and was looking over a few different similar options on Amazon. I did look outside of amazon as well, but Amazon had the best prices in my opinion. I was hesitant on each selection due to either pricing or reviews that scared me away. I ended up going with this one since they were offering a deal on the red and black one - as soon as I saw that, I was sold! I believe it was a middle of the pack price for comparison.
Overall, I really like the chair, but it does come with one warning I will share below:
**DELIVERY** 10/10
I ordered this chair and though I think it did say it was PRIME eligible, it showed that the delivery as a future date that was about two weeks out. I am impatient and entitled, much like most of us Primers, haha, but due to the deal, I bit the bullet and ordered and made myself forget about it since it would be a bit. MUCH to my surprise, it showed up a few days later at my doorstep!!! I was really excited!!! A+ on this category!
**PACKAGING** 9/10
After reading some reviews that stated that the packing was rough, I'm not going to lie, I was expecting a mangled box to be at my front door. Also to my surprise, the box was perfectly fine! Maybe this was feedback that the distributor took and made the necessary changes? I don't know, but everything arrived undamaged in terms of the box. I deducted one point because one of the armrests (left side) had a "chunk" that lifted off the top a bit. To try and describe it, the armrests are like a hard foam, and best I can describe, it was as if something nicked the top flat surface of it and peeled back a small chunk, about the size of a pea. I pushed it back down and it sat. I forgot about it until writing this, and now cannot find it.
**ASSEMBLY** 8/10
Assembly took about 45 minutes to an hour or so. It could have gone faster, but the screw hole on the back rest was crooked, so when attaching the back rest to the seat with the supplied bracket, I kept having trouble and eventually had to remove the back rest completely to try and see what was going on. The screw hole was slightly tilted which was making it hard to thread. Once I saw what was going on, I was about to put the screw in with that in mind and just came in at a slight angle. Everything else was pretty straight forward!
**LOOKS** 9/10
This chair looks really cool. That said, I am in to cars and making them fast/cool, so I do have a bias. The only thing I don't care for as much are the wheels - as much as I love making cars look cool, I thought it was a little corny for computer chair wheels, lol. I don't notice it as much now that it has been assembled, though.
**FUNCTION** 10/10 with one warning
As I said, my old chair sucked, so this is in comparison with that chair. When it comes to function, this hit all the boxes for me. The material is soft and comfortable. The seat cushion is good for me, but I am 145lbs, so not sure how it would fair for a large individual. The chair is pretty wide so I think it would fit a larger person just fine, plus the arm rests can swivel and lock into three different positions - straight, pointed outwards, pointed inwards. Having them pointed outwards allows me to rest my elbows on the backs of them while gaming or working. The back support piece is SUPER helpful for me. It can be re-positioned easily and stays in place. The headrest is nice for neck support, but doesn't stay in place as well and can sag down, but not a big deal.
The warning I was talking about is actually a perk of functioning, but not for me in one instance. Yes, the chair reclines all the way back. Even better, it doesn't tip while doing it, which I thought it surely would laid all the way back. The problem for me is that I was on a work zoom meeting that didn't require the camera on, I reclined all the way back and was "listening." I woke up later :-/
Good chair, I would recommend it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 3, 2020
★★★★★ 5
Outstanding Chair
Color: Matte-black
This is an outstanding chair.
It’s very well built and has handled up to 300 lbs. with no issues after a couple of weeks of use. The engineering is excellent, the assembly was straightforward, and everything fit together exactly as it should. No stripped bolts, no misaligned holes—something anyone who has assembled other furniture will appreciate.
Overall, it’s sturdy, well-designed, and extremely comfortable. The adjustable armrests are a standout feature and make a big difference in day-to-day use.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 21, 2026
recommand products
Gates 14-18 Jeep Cherokee DriveAlign Automatic Belt Tensioner - 39342
78.36
Turbosmart eB2 High Pressure Hose Fitting Kit - TS-0301-2020
77.39
Clutch Masters 89-94 Eagle Talon 2.0L Non-Turbo FX250 Clutch Kit - 05051-HD0F
265.62
EBC 2021+ BMW M3/M4 3.0TT (G80/G82/G83) Bluestuff Rear Brake Pads - DP52089NDX
157.59
EBC 06-09 Pontiac Solstice / 06-10 Saturn Sky Bluestuff Front Brake Pads - DP51763NDX
115.53