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Description
Rat RAP2B ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the Ras-related protein Rap-2b (RAP2B). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Ras-related protein Rap-2b (RAP2B) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Ras-related protein Rap-2b ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RAP2B (RAP2B), also known as Ras-associated protein Rap-2b, is a member of the RAS oncogene family. It is encoded by the RAP2B gene. It belongs to the Ras-associated protein family. This intronless gene belongs to the RAS-related gene family. The proteins encoded by these genes share approximately 50% amino acid identity with classical RAS proteins and share many structural features. The most significant difference between RAP and RAS proteins is their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 1258 reviews
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Product Reviews
★★★★★ 5
Well designed, secure hold
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
This Tesla screen phone mount feels well designed and has a strong MagSafe magnet to hold your phone. It is meant for the newer generation of Teslas with the screen that sits out from the instrument panel. It’s sits on the corner and has padding to keep it from scratching the screen. The size of the grippers keeps them right at the edge of the bevel so it doesn’t block any portion of the screen. They are easily tightened with a rotating dial that doesn’t slip. The MagSafe magnet can be positioned in a variety of positions. The kit comes with an adhesive metal ring in case your phone or case isn’t MagSafe compatible. Unfortunately, it didn’t come with any instructions, but it was easy enough to figure out.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 23, 2025
★★★★★ 4
Mount your MagSafe compatible phone to the Tesla monitor
Style: MagSafe Car Phone Holder
The Tesla MagSafe monitor mount fits the screen of a Model 3 perfectly. It has a strong magnet and when you attach it to the monitor and tighten the wheels this mount stays put. I can now have my phone docked next to the monitor in front of the steering wheel, which really helps me unlock the phone when I need to access things like my phone's calendar or emails. I can also use the phone as a music switching interface when it's connected to the car via bluetooth.
The design of the mount is very simple, it has two cushioned clamps, I never felt like I was compressing the clamps too hard that it'll crack the screen but I just adjusted it enough so it won't fall down with the weight of the phone on the mount. The magnet of the mount is very strong and as long as your phone supports MagSafe docking it will not have a risk of falling when you drive even over bumps. The arm of the mount is adjustable so you can place it at the appropriate angle that you like.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 29, 2025
★★★★★ 5
Good phone mount
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
So far I’m very happy with the phone mount for my Tesla Model 3. It installed very easy, feels secure, the magnetic ring holds my iPhone really well. My last mount had an issue with staying in place, the phone would often tilt when going over bumps but this one seems solid. There were no installation instructions but it was a no brainer to install. Like most mounts the give you a separate metal ring you can attach to the back of your phone if your phone or case isn’t MagSafe.
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Reviewed in the United States on October 22, 2025
★★★★★ 4
Made of alloy and mostly plastic
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
This is a fairly decent MagSafe mount that attaches cleanly to an LCD car display. It works and looks good on my Tesla Model 3. The only part that is metal is the bracket but the rest seems to be made from a metallic looking plastic. I do like that the inside of the clamps can be adjusted independently and there’s a layer of silicone padding to protect the screen. I was able to make it pretty tight with no movement at all.
It only works well on a “naked” phone or a cover that has MagSafe ring built in. I have a super thin case and it seems to hold pretty well so far but I do wish the magnets were a bit stronger.
The good thing is that it uses a standard ball joint so I have other types of mounts that could easily fit in place of the MagSafe version.
The only other thing that I think detracts from the overall “clean look” is the screened text on the clamp that simply says “Screen Holder”. It would have been better not there.
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Reviewed in the United States on October 24, 2025
★★★★★ 5
EXCELLENT Product for using with the dash mount BUT not so good for all cars with the vent mount.
This is a great product for wireless phones worked perfectly for my SG S21 the mount is really good sturdy flexible and firm if done right. BUT the vent jack plug is short for my Camry 2018 doesn't hold the phone firmly. It keeps wobbling when driving. So if you're looking for using just the vent holder make sure your car has a short AC flap so it can mount the plug firmly hold the phone properly. The phone holder is amazing it works really good just the way it is shown in the description. So guys first check the flap size of your cars AC vent must be short if you're looking to use the Vent plug holder. But if you're going to use it only with the dash mount its fantastic and holds the phone really firm and good. I really love this product and highly recommend to buy.
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Reviewed in the United States on September 12, 2025
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