UA-Glo® Luminescent Kinase Assay
SKU: 95380287749

UA-Glo® Luminescent Kinase Assay

Sale price$74.70 Regular price$83.00
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 6 - Jul 11

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

UA-Glo® Luminescent Kinase AssayProduct Specification Stability & Storage Dry ice transportation. Store in the dark at 20C or below, with a validity period of 12 months. Background The UA Glo Kinase Luminescent Detection Kit can quantitatively determine the activity of kinases in enzymology experiments. This kit measures kinase activity by quantitatively detecting the amount of remaining ATP after the kinase reaction: the amount of remaining ATP is negatively correlated with kinase

Product Specification


Stability & Storage

Dry ice transportation. Store in the dark at -20°C or below, with a validity period of 12 months.

Background

The UA-Glo Kinase Luminescent Detection Kit can quantitatively determine the activity of kinases in enzymology experiments. This kit measures kinase activity by quantitatively detecting the amount of remaining ATP after the kinase reaction: the amount of remaining ATP is negatively correlated with kinase activity. When using this kit, the maximum ATP concentration in the kinase reaction can reach 10μM; when using the UA-Glo Kinase Plus Luminescent Detection Kit, the maximum ATP concentration in the kinase reaction can reach 100μM; and when using the UA-Glo Kinase Max Luminescent Detection Kit, the maximum ATP concentration in the kinase reaction can reach 500μM. This kit can detect most kinases, and kinase substrates include polypeptides, proteins, lipids, or sugars, etc. The kit can be used for high-throughput homogeneous screening of kinase inhibitors, and can distinguish between ATP-competitive and non-competitive inhibitors by adjusting the ATP concentration.

Components

Product composition: Luciferase, luciferin, and buffer are mixed and filled into 10 ml or 100 ml brown bottles. The specifications are as follows:

Specification

96-well plate reaction count

384-well plate reaction count

10 ml

200

1,000

100 ml

2,000

10,000

10 x 100 ml

20,000

100,000

Protocol

1. Kinase Reaction
1)Perform the kinase reaction in a 96-well or 384-well white opaque assay plate. It is recommended that the reaction volume is 50μL for 96-well plates and 10μL for 384-well plates. Test compounds with concentration gradients can be added to the kinase reaction.
2) The concentrations of kinase and substrate in the kinase reaction need to be optimized according to different kinases. Under the condition of the appropriate signal-to-noise ratio required for the experiment, the kinase concentration within the linear reaction range of the signal can be used for the experiment.
3) Concentration of ATP: The UA-Glo Kinase Luminescent Detection Kit can be used up to 10μM.
4) The kinase reaction can be carried out in a universal reaction buffer (40mM Tris-HCl (pH 7.5), 0.1 mg/ml BSA, 20mM MgCl2) or using reaction buffers and cofactors reported in the literature.
5) The temperature and time of the kinase reaction need to be set according to different kinases. For high-throughput screening experiments of compounds, it is recommended to optimize the kinase reaction to be carried out at room temperature (22℃-25℃) to help maintain the temperature uniformity of the assay plate during the kinase activity determination.

2.  Kinase Activity
1) Take out the UA-Glo Kinase Luminescent Detection Reagent and equilibrate it to room temperature. Mix gently by shaking. The luciferase reaction in the kinase activity luminescent detection reagent is sensitive to temperature changes; the reagent and assay plate need to be equilibrated to room temperature, and the temperature should be kept constant (±1℃) during the test.
2) If the kinase reaction is carried out at a non-room temperature, such as 30℃, the assay plate needs to be equilibrated to room temperature.
3) Add 50 µL of kinase luminescent detection reagent to a 50 µL 96-well assay plate, or 10 µL of kinase luminescent detection reagent to a 10 µL 384-well assay plate, shake to mix, and place in the dark for 10 minutes.
4) Read the fluorescence signal. Since the fluorescence signal is very stable, if necessary, the plate can be read within 3 hours after adding the detection reagent.

Guidelines

1) It is not recommended to arbitrarily change the amount of reaction reagents without strict verification. The volume ratio of the kinase reaction reagent to the UA-Glo kinase luminescent detection reagent should be 1:1.

2) After the first use of the UA-Glo kinase luminescent detection reagent, it should be aliquoted and stored at -20°C to ensure the stability of the reagent. There is no loss of signal intensity after 4 repeated freeze-thaw cycles.

3) This product is for research use only.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 95380287749

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.3 ★★★★★
Based on 1871 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
B
Verified Purchase
Boots
Louisville, US
★★★★★ 5
Great Product
Just as described and prompt shipping
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 18, 2026
L
Verified Purchase
larry barnett
Draper, US
★★★★★ 5
They are great
Work as they should.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 7, 2026
D
Verified Purchase
david balderrama
Belleville, US
★★★★★ 5
Good price!
These are your basic filters for jeep or Chrysler vehicles with the regular oil change interval mileage. Cheaper than Autozone for the price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 17, 2025
R
Verified Purchase
Riggs
Pawtucket, US
★★★★★ 5
Fits my 2026 civic sport perfect!
Size: AF151
No issues, fitment is perfect. Great quality for an excellent price. Dealer wanted like $24 for a basic one. Nah I'm good I'll just go on Amazon and get it for $8. Took 30 seconds from start to finish can't get any easier.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
A
Verified Purchase
Ant Farmer 1
Draper, US
★★★★★ 5
Fits well and installs easily.
Size: AF997
This filter looked about the same as the OEM filter. It installed in about a minute. The quality appeared to be fine. It was protected in a cardboard box. It fit my 2014 Subaru Outback perfectly. I just installed it an hour ago so I don’t have any long term observations about longevity or effectiveness. I can’t see any reason why it would not work as well as the original, it seemed identical.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026

recommand products