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Description
LIF Protein, Human (GMP)Product Specification Species Human Synonyms Leukemia inhibitory factor; LIF; Differentiation stimulating factor (D factor); Melanoma derived LPL inhibitor (MLPLI); Emfilermin; Accession P15018 Amino Acid Sequence Ser23 Phe202 Expression System E. coli Mycoplasma Sterility phValue 7. 8~8. 2 ProteinContent 90%~120% SdsPageMolecularWeight 202kD SdsPagePurity 97% RpHplcPurity 95% SecHplcPurity 97% CellActivity < 0. 3ng ml Conjugation Unconjugated Tag No
Product Specification
| Species | Human |
| Synonyms | Leukemia inhibitory factor; LIF; Differentiation-stimulating factor (D factor); Melanoma-derived LPL inhibitor (MLPLI); Emfilermin; |
| Accession | P15018 |
| Amino Acid Sequence | Ser23-Phe202 |
| Expression System | E.coli |
| Mycoplasma | 阴性 |
| Sterility | 符合 |
| phValue | 7.8~8.2 |
| ProteinContent | 90%~120% |
| SdsPageMolecularWeight | 20±2kD（还原） |
| SdsPagePurity | ≥97%（非还原） |
| RpHplcPurity | ≥95% |
| SecHplcPurity | ≥97% |
| CellActivity | < 0.3ng/ml |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Development. 2005 Mar;132(5):885-96. |
Background
Leukemia Inhibitory Factor (LIF) is a member of the IL-6 family of cytokines. It is a multifunctional glycoprotein with a molecular weight ranging from 28 to 45 kDa, depending on the expression system and post-translational modifications. LIF is composed of a signal peptide, an extracellular domain, and an intracellular domain, which collectively endow it with diverse biological functions. LIF is expressed in various tissues, including the liver, lungs, heart, nervous system, and immune cells, highlighting its widespread distribution and significance.
LIF is a critical cytokine widely used in biomedical research, particularly in stem cell biology and regenerative medicine. It also plays a crucial role in maintaining the pluripotency and inducing the differentiation of stem cells, the treatment of neurodegenerative diseases, the modulation of inflammatory responses.
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